Isolation and culture of endothelial cells from mouse aorta preparation of aorta rings 1.
Mouse primary aortic endothelial cells.
The vascular endothelial cells play a significant role in many important cardiovascular disorders.
Primary human aortic endothelial cells acbri 375 were initiated from normal human descending aorta aortic arch to renal artery.
Paul huang cardiovascular.
Cut off tissue to open chest and abdomen and expose aorta.
Isolation of mouse primary aortic endothelial cells by selection with specific antibodies methods mol biol.
Authors pedro molina sánchez 1 vicente andrés 2 affiliations 1 laboratory of molecular and genetic.
Mouse aortic endothelial cells maec were isolated from the thoracic and abdominal aortas of various control and knockout mouse lines.
Endothelial cells ecs are key blood vessel wall components that play critical roles in the regulation of many physiological processes including angiogenesis coagulation and vascular tone.
C57bl 6 mouse primary aortic endothelial cells can be used in assays of cell cell adhesion migration vascular tube formation or transendothelial resistance ter.
Wipe off skin with 70 isopropanol and fix mice on an operation table.
Isolation of mouse primary aortic endothelial cells by selection with specific antibodies springerlink.
Previously reported procedures for the isolation of ec have required magnetic beads or fluorescence activated cell sorting facs to purify the cells.
Mouse aortic endothelial cells were isolated from c57bl 6 mice and used for experiments without any passage.
Enos knockout mice were kindly provided by dr.
Marek drab max planck institute for molecular cell biology and genetics dresden germany.
This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment.
Standard biochemical procedures performed with endothelial cell cultures include rt pcr western blotting immunoprecipitation immunofluorescent staining immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Wild type c57bl 6 and p47 phox knockout mice were purchased from jackson laboratories bar harbor maine.
Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
Primary human aortic endothelial cells or haoecs acbri 375 were initiated from normal human descending aorta aortic arch to renal artery.
Caveolin 1 cav1 knockout mice were kindly provided by dr.
Method of primary cell culture with appropriate reagents.
Mouse aortic endothelial cells maoec line the vessel wall of aorta and are constantly exposed to high hemodynamic forces.
The human umbilical vein endothelial cells were cultured by using cell passageway to further growth in the culture medium.
Euthanatize mice by co 2 asphyxiation followed by cervical dislocation.
